Or_Grow's Tissue Culture Journal
Posted: Fri Jun 21, 2019 3:40 pm
Hoping to demonstrate that any competent grower can do tissue culture work, enabling low-cost, small footprint storage and plant trading of in vitro plants, among other things.
The end goal is this type of thing:
But, this initial project is to brush up on my sterile techniques using an in vitro tissueponics kit by Microclone.
I have a HEPA Laminar-flow setup, but wanted to demo that a hepa-filtered fan like this can handle the job in a small room or tent: Equip & Supplies were sterilized in a pressure cooker @ 15-20psi for 20 minutes: Process was done in a 4x4: Cuttings soaking in 500ppm chlorine solution
Around day21, I will be cutting up these plants, to prep them for in vitro replication, then in vitro rooting, then transplant to ex vitro rr's for acclimitization...I'll be using another Microclone kit for this work.
Cutting placed in sugar-hormone-antimicrobial solution The cuttings were placed in a 2x3x4.5 Running under an incandescent light (for heat) and a hlg96elite, running at:
Here is what they look like today 6/21, Day 9, ~77F and ~75% rh
The end goal is this type of thing:
But, this initial project is to brush up on my sterile techniques using an in vitro tissueponics kit by Microclone.
I have a HEPA Laminar-flow setup, but wanted to demo that a hepa-filtered fan like this can handle the job in a small room or tent: Equip & Supplies were sterilized in a pressure cooker @ 15-20psi for 20 minutes: Process was done in a 4x4: Cuttings soaking in 500ppm chlorine solution
Around day21, I will be cutting up these plants, to prep them for in vitro replication, then in vitro rooting, then transplant to ex vitro rr's for acclimitization...I'll be using another Microclone kit for this work.
Cutting placed in sugar-hormone-antimicrobial solution The cuttings were placed in a 2x3x4.5 Running under an incandescent light (for heat) and a hlg96elite, running at:
Here is what they look like today 6/21, Day 9, ~77F and ~75% rh