Or_Grow's Tissue Culture Journal

The fruits of our labor. We welcome all types of plants, but grows posted here must be legal.
Or_Gro
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Day 13: No contam, they’re still alive w leaves growing; another week or so and they are going into replication mode:
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LEDG
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I have the same hygrometer. Solid purchase.
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Or_Gro
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Likin these donut humidifiers, in a 2x3x4.5’:
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Two styles:
B1A007C8-BCBC-4E97-846A-BE742145E5E1.jpeg [attachment=1]903BF758-FE2F-4F2B-998F-0CE361E66FCB.jpeg
Attachments
B1A007C8-BCBC-4E97-846A-BE742145E5E1.jpeg
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Or_Gro
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Or_Gro wrote:
Tue Jun 25, 2019 3:15 pm
Likin these donut humidifiers, in a 2x3x4.5’:

85715F5A-ACC7-46A0-B57C-B9ADE0A85144.jpeg

Two styles:

B1A007C8-BCBC-4E97-846A-BE742145E5E1.jpeg

[attachment=1]903BF758-FE2F-4F2B-998F-0CE361E66FCB.jpeg
I bought units of both models from diff vendors, all of each style seem to be exactly the same, so go for least expensive one for each style.

The long-stem version seems to have some type of built in timer...haven’t spent the time to determine the cycle length...so, it needs monitoring.

The floater seems to put out more humidity, this one needs a timer to hold rh at your desired level.

I typically use 4L ultrasonics in my larger tents, will substitute one of these in my 3x3 with mama...see what happens.

My main question is how long do they last?

Btw, i’m using 220ppm tapwater, instructions say DON’T use distilled water....lots of back-n-forth on the interwebs about using tap vs distilled water in ultrasonics...many instances where ultrasonics don’t work well with low/no ppm water...
Or_Gro
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LEDG wrote:
Tue Jun 25, 2019 2:58 pm
I have the same hygrometer. Solid purchase.
I like them...

but rh meter seems to be the weak link, have a bunch of them that lost accuracy over a year of use in high rh conditions...
Or_Gro
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Here’s an overview of tissue culture from a professional’s perspective:



Here’s what i’m doing:
Or_Gro
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Day 14: root callus/bumps showing
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Cannabis callus/root bumps
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Grape roots
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Or_Gro
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Day 15:
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Or_Gro
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Day 16:
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Or_Gro
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Day 17:
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